PCCs are designed to bind to and detect designated motifs (epitopes) on target proteins. They are developed by using a designated region (the epitope) of the protein target as the catalytic scaffold for assembling the PCC. This epitope-directed process lends exquisite selectivity to the PCC. The small size of the resulting molecule – approximately one-fortieth the molecular weight of an equivalent monoclonal antibody – suggests a new class of diagnostic and therapeutic agents that resembles a peptide therapeutic with antibody-like binding properties.

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Reference Papers

Inhibition of Heme Sequestration of Histidine‐Rich Protein 2 Using Multiple Epitope‐Targeted Peptides

JingXin Liang, David N. Bunck, Anvita Mishra, Sunga Hong, Matthew N. Idso, James R. Heath 
Journal of Peptide Science, 2019, DOI: 10.1002/psc.3203

Protein-Catalyzed Capture Agents

Heather D. Agnew, Matthew B. Coppock, Matthew N. Idso, Bert T. Lai, JingXin Liang, Amy M. McCarthy-Torrens, Carmen M. Warren, James R. Heath 
Chemical Reviews, 2019, DOI: 10.1021/acs.chemrev.8b00660

An Allosteric Inhibitor of KRas Identified Using a Barcoded Rapid Assay Microchip Platform

Amy Michelle McCarthy, Jungwoo Kim, Anna Katrine Museth, Ryan K. Henning, John E. Heath, Emma Winson, Joseph J. Oh, JingXin Liang, Sunga Hong, James R. Heath 
Analytical Chemistry, 2018, 90 (15), pp 8824-8830

Modulating the Folding Landscape of Superoxide Dismutase 1 with Targeted Molecular Binders

David N. Bunck, Beatriz Atsavapranee, Anna K. Museth, David VanderVelde, James R. Heath 
Angewandte Chemie International Edition, 2018, 57 (21), pp 6212-6215

Epitope Targeted Macrocyclic Peptide Ligand with Picomolar Cooperative Binding to Interleukin-17F

Bert T. Lai, Jeré A. Wilson, Jacquie Malette Loredo, Suresh M. Pitram, Nicole A. LaBerge, James R. HeathHeather D. Agnew
Chemistry - A European Journal, 2018, 24 (15), pp 3760-3767

Protein Catalyzed Capture Agents with Tailored Performance for In Vitro and In Vivo Applications

Matthew B. Coppock, Candice R. Warner, Brandi Dorsey, Joshua A. Orlicki, Deborah A. Sarkes, Bert T. Lai, Suresh M. Pitram, Rosemary D. Rohde, Jacquie Malette, Jeré A. Wilson, Paul Kearney, Kenneth C. Fang, Scott M. Law, Sherri L. Candelario, Blake Farrow, Amethist S. Finch, Heather D. AgnewJames R. Heath, and Dimitra N. Stratis-Cullum
Peptide Science, 2017, 108, e22934

Degradation of Akt Using Protein-Catalyzed Capture Agents

Ryan K. Henning, Joseph O. Varghese, Samir Das, Arundhati Nag, Grace Tang, Kevin Tang, Alexander M. Sutherland and James R. Heath
Journal of Peptide Science, 2016, 22 (4), pp 196-200

A General Synthetic Approach for Designing Epitope Targeted Macrocyclic Peptide Ligands

Samir Das, Arundhati Nag, JingXin Liang, David N. Bunck, Aiko Umeda, Blake Farrow, Matthew B. Coppock, Deborah A. Sarkes, Amethist S. Finch, Heather D. Agnew, Suresh Pitram, Bert Lai, Mary Beth Yu, A. Katrine Museth, Kaycie M. Deyle, Bianca Lepe, Francis P. Rodriguez-Rivera, Amy McCarthy, Belen Alvarez-Villalonga, Ann Chen, John Heath, Dimitra N. Stratis-Cullum, James R. Heath
Angewandte Chemie International Edition, 2015, 54 (45), pp 13219–13224

Epitope Targeting of Tertiary Protein Structure Enables Target-Guided Synthesis of a Potent In-Cell Inhibitor of Botulinum Neurotoxin

Blake Farrow, Michelle Wong, Jacquie Malette, Dr. Bert Lai, Dr. Kaycie M. Deyle, Dr. Samir Das, Dr. Arundhati Nag, Dr. Heather D. Agnew and Prof. James R. Heath
Angewandte Chemie International Edition, 2015, 54 (24), pp 7114–7119

A Protein-Targeting Strategy Used to Develop a Selective Inhibitor of the E17K Point Mutation in the PH Domain of Akt1

Kaycie M. Deyle, Blake Farrow, Ying Qiao Hee, Jeremy Work, Michelle Wong, Bert Lai, Aiko Umeda, Steven W. Millward, Arundhati Nag, Samir Das, and James R. Heath
Nature Chemistry, 2015, 7, pp 455–462

A Chemical Epitope-Targeting Strategy for Protein Capture Agents: The Serine 474 Epitope of the Kinase Akt2

Arundhati Nag, Samir Das, Mary Beth Yu, Kaycie M. Deyle, Steven W. Millward, and James R. Heath
Angewandte Chemie International Edition, 2013, 52 (52), pp 13975-13979

A Chemically Synthesized Capture Agent Enables the Selective, Sensitive, and Robust Electrochemical Detection of Anthrax Protective Antigen

Blake Farrow, Sung A Hong, Errika C. Romero, Bert Lai, Matthew B. Coppock, Kaycie M. Deyle, Amethist S. Finch, Dimitra N. Stratis-Cullum, Heather D. Agnew, Sung Yang, and James R. Heath
ACS Nano, 2013, 7 (10), pp 9452–9460

A Cocktail of Thermally Stable, Chemically Synthesized Capture Agents for the Efficient Detection of Anti-Gp41 Antibodies from Human Sera

Jessica A. Pfeilsticker, Aiko Umeda, Blake Farrow, Connie L. Hsueh, Kaycie M. Deyle, Jocelyn T. Kim, Bert T. Lai, and James R. Heath
PLoS ONE, 2013, 8 (10), e76224

In Situ Click Chemistry: From Small Molecule Discovery to Synthetic Antibodies

S. W. Millward, H. D. Agnew , B. Lai, S. S. Lee, J. Lim, A. Nag, S. Pitram, R. Rohde, and J. R. Heath
Integrative Biology, 2013, 5, pp 87–95

Iterative in Situ Click Chemistry Assembles a Branched Capture Agent and Allosteric Inhibitor for Akt1

Steven W. Millward, Ryan K. Henning, Gabriel A. Kwong, Suresh Pitram, Heather D. Agnew, Kaycie M. Deyle, Arundhati Nag, Jason Hein, Su Seong Lee, Jaehong Lim, Jessica A. Pfeilsticker, K. Barry Sharpless, and James R. Heath
Journal of the American Chemical Society, 2011, 133 (45), pp 18280–18288

Iterative In Situ Click Chemistry Creates Antibody-like Protein-Capture Agents

Heather D. Agnew, Rosemary D. Rohde, Steven W. Millward, Arundhati Nag, Woon-Seok Yeo, Jason E. Hein, Suresh M. Pitram, Abdul Ahad Tariq, Vanessa M. Burns, Russell J. Krom, Valery V. Fokin, K. Barry SharplessJames R. Heath
Angewandte Chemie International Edition, 2009, 48 (27), pp 4944–4948